Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostic
This book reflects the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Fluorescence lifetime approaches maximize contrast between different fluorophores for improved specificity of measurements, enabling characterization of intact tissue and disease diagnostics. The book offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation.