Molecular Biology of the Gene 5e PIE
Every student package automatically includes a CD-ROM and access to a website containing tutorials, molecular models, and more.
The long-awaited Fifth Edition of James D. Watson's classic text, Molecular Biology of the Gene , has been thoroughly revised and is published to coincide with the 50th anniversary of Watson and Crick's paper on the structure of the DNA double-helix.
Though completely updated, the new edition retains the distinctive character of earlier editions that made it the most widely used book in molecular biology. Twenty-one concise chapters, co-authored by five highly respected molecular biologists, provide current, authoritative coverage of a fast-changing discipline. The completely new art is printed in full color for the first time.
Divided into five parts, the first (Chemistry and Genetics) begins with an overview of molecular biology, placing the discipline in historical context and introducing the basic chemical concepts that underpin our description of molecular biology today. The second and third parts (Maintenance of the Genome and Expression of the Genome) form the heart of the book, describing in detail the basic mechanisms of DNA replication, transcription and translation. The fourth part of the book (Regulation) deals with how gene expression is regulated - from the examination of basic mechanisms that regulate gene expression in bacterial and eukaryotic systems, to a description of how regulation of gene expression lies at the heart of the process of development. Recent findings from sequencing whole genomes of several animals have revealed that they all share essentially the same genes. The last chapter in the regulation section looks at how changes in gene regulation can account for how different animals can be made up of the same genes. The final part of the book (Methods) deals with the techniques and methods used in molecular biology.
Table of Contents
I. CHEMISTRY AND GENETICS.
1. The Mendelian View of the World.
Chromosomal Theory of Heredity.
Gene Linkage and Crossing Over.
The Origin of Genetic Variability Through Mutations.
Early Speculations about What Genes Are and How They Act.
Preliminary Attempts to Find a Gene-Protein Relationship.
2. Nucleic Acids Convey Genetic Information.
Avery's Bombshell: DNA Can Carry Genetic Specificity.
The Double Helix.
The Genetic Information within DNA is Conveyed by the Sequence of its Four Nucleotide Building Blocks.
The Central Dogma.
Establishing the Direction of Protein Synthesis.
The Era of Genomics.
3. The Importance of Weak Chemical Interactions.
Characteristics of Chemical Bonds.
The Concept of Free Energy.
Weak Bonds in Biological Systems.
4. The Importance of High-Energy Bonds.
Molecules that Donate Energy Are Thermodynamically Unstable.
Enzymes Lower Activation Energies in Biochemical Reactions.
High-Energy Bonds in Biosynthetic Reactions.
Activation of Precursors in Group Transfer Reactions.
5. Weak and Strong Bonds Determine Macromolecular Structure.
Higher-Order Structures Determined by Intra- and Intermolecular Interactions.
The Specific Conformation of a Protein Results from its Pattern of Hydrogen Bonds.
Most Proteins Are Modular, Containing Two or Three Domains.
Weak Bonds Correctly Position Proteins Along DNA and RNA Molecules.
Allostery: Regulation of a Protein's Function by Changing its Shape.
II. MAINTENANCE OF THE GENOME.
6. The Structures of DNA and RNA.
7. Chromosomes, Chromatin, and the Nucleosome.
Chromosome Sequence and Diversity.
Chromosome Duplication and Segregation.
Higher-Order Chromatin Structure.
Regulation of Chromatin Structure.
8.The Replication of DNA.
The Chemistry of DNA Synthesis.
The Mechanism of DNA Polymerase.
The Replication Fork.
The Specialization of DNA Polymerases.
DNA Synthesis at the Replication Fork.
Initiation of DNA Replication.
Binding and Unwinding: Origin Selection and Activation by the Initiator Protein.
9. The Mutability and Repair of DNA.
Replication Errors and Their Repair.
Repair of DNA Damage.
10. Homologous Recombination at the Molecular Level.
Models for Homologous Recombination.
Homologous Recombination Protein Machines.
Homologous Recombination in Eukaryotes.
Genetic Consequences of the Mechanism of Homologous Recombination.
11. Site-Specific Recombination and Transposition of DNA.
Conservative Site-Specific Recombination.
Biological Roles of Site-specific Recombination.
Examples of Transposable Elements and their Regulation.
III. EXPRESSION OF THE GENOME.
12. Mechanisms of Transcription.
RNA Polymerases and the Transcription Cycle.
The Transcription Cycle in Bacteria.
Transcription in Eukaryotes.
13. RNA Splicing.
The Chemistry of Splicing.
The Spliceosome Machinery.
14. Translation 411.
Attachment of Amino Acids to tRNA.
Initiation of Translation.
Termination of Translation.
Translation-Dependent Regulation of mRNA and Protein Stability.
15. The Genetic Code.
The Code Is Degenerate.
Three Rules Govern the Genetic Code.
Suppressor Mutations Can Reside in the Same or a Different Gene.
The Code Is Nearly Universal.
16. Gene Regulation in Prokaryotes.
Principles of Transcriptional Regulation.
Regulation of Transcription Initiation: Examples from Bacteria.
Examples of Gene Regulation at Steps after Transcription Initiation.
The Case of Phage ?: Layers of Regulation.
17. Gene Regulation in Eukaryotes.
Conserved Mechanisms of Transcriptional Regulation from Yeast to Mammals.
Recruitment of Protein Complexes to Genes by Eukaryotic Activators.
Signal Integration and Combinatorial Control.
Signal Transduction and the Control of Transcriptional Regulators.
Gene “Silencing” by Modification of Histones and DNA.
Eukaryotic Gene Regulation at Steps after Transcription Initiation.
RNAs in Gene Regulation.
18. Gene Regulation During Development.
Three Strategies by Which Cells Are Instructed to Express Specific Sets of Genes During Development.
Examples of the Three Strategies for Establishing Differential Gene Expression.
The Molecular Biology of Drosophila Embryogenesis
19. Comparative Genomics and the Evolution of Animal Diversity.
Most Animals Have Essentially the Same Genes.
Three Ways Gene Expression Is Changed During Evolution.
Experimental Manipulations that Alter Animal Morphology.
Morphological Changes in Crustaceans and Insects.
Genome Evolution and Human Origins.
20. Techniques of Molecular Biology.
21. Model Organisms.
Baker's Yeast, Saccharomyces cerevisiae.
The Nematode Worm, Caenorhabditis elegans.
The Fruit Fly, Drosophila melanogaster.
The House Mouse, Mus musculus.